Rat Cytochrome P450 1A1 (CYP1A1) ELISA Kit from MyBioSource.com

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Rat Cytochrome P450 1A1 (CYP1A1) ELISA Kit

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Description

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Nesfatin1 (also known as CYP1A1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat CYP1A1 antibody. CYP1A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CYP1A1 Antibody is added and binds to CYP1A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYP1A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CYP1A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm